Journal: Technology in Cancer Research & Treatment

Article Title: The Expression Characteristics of the RBFOX1 Gene in Colorectal Cancer

doi: 10.1177/15330338251333695

Figure Lengend Snippet: Copy Number Variation of RBFOX1 and Its Correlation with DFS and OS. A. Online Data from Cbioportal shows that RBFOX1 has the Highest Frequency of Copy Number Deletions in Colorectal Cancer. B And C. Analysis of RBFOX1 Copy Number Situation in Colorectal Adenocarcinoma (TCGA, Firehose Legacy) and Colon Cancer (CPTAC-2 Prospective, Cell 2019) Data. D. KM Survival Curve of Progression-Free Survival and Overall Survival Among the Four Groups.

Article Snippet: A2BP1 Polyclonal antibody (Proteintech, USA, 22647-1-AP) was added dropwise and incubated at 37 °C for 1 h. The liquid was poured off, and the sections were rinsed with PBS three times.

Techniques:

Journal: Technology in Cancer Research & Treatment

Article Title: The Expression Characteristics of the RBFOX1 Gene in Colorectal Cancer

doi: 10.1177/15330338251333695

Figure Lengend Snippet: Characteristics of RBFOX1 mRNA in Colorectal Cancer. A. Correlation Analysis of Total RBFOX1 Copy Number Variation and RBFOX1 mRNA Expression. B. Differences in RBFOX1 mRNA Expression Among the Four Groups, with Significant Differences Between Groups A And C (**** Represent P < 0.0001), and Significant Differences Between Groups C And D (*** Denotes P < 0.001). C-F. Correlation Analysis of Copy Number Variation and Corresponding mRNA Expression in Each Group Among the Four Groups., C: (Group A–High–Level Copy Number Deletions Group), D(Group B– Represent Low-Level Deletion Group), E(Group C– Normal Group), F(Group D –Copy Number Amplification Group). G. Relative mRNA Expression of RBFOX1 in Colon Adenocarcinoma and Rectal Adenocarcinoma, Indicating that RBFOX1 mRNA Expression in Colorectal Cancer is Lower than in Normal Tissues. H. Correlation between BRAF Gene Mutation and RBFOX1 mRNA in Colon Cancer and Rectal Cancer Tissues. I. Correlation Between PIK3CA Gene Mutation and RBFOX1 mRNA in Colon Cancer and Rectal Cancer Tissues.

Article Snippet: A2BP1 Polyclonal antibody (Proteintech, USA, 22647-1-AP) was added dropwise and incubated at 37 °C for 1 h. The liquid was poured off, and the sections were rinsed with PBS three times.

Techniques: Expressing, Amplification, Mutagenesis

Journal: Technology in Cancer Research & Treatment

Article Title: The Expression Characteristics of the RBFOX1 Gene in Colorectal Cancer

doi: 10.1177/15330338251333695

Figure Lengend Snippet: Immunofluorescence Analysis of RBFOX1 Protein in Tumor Cells. A. Immunofluorescence from the Human Protein Atlas Database shows that RBFOX1 has Different Nuclear-Cytoplasmic Distribution Characteristics in Cancer Cells of Three Different Tissues. ( https://www.proteinatlas.org/search/RBFOX1 ) B. COSMIC Cancer Cell Engineering shows Copy Number Deletions in Multiple Colorectal Cancer Cells. C. Immunofluorescence shows that RBFOX1 Protein is Mainly Distributed in the Nucleus of Well-Differentiated Tumor Cells HT-29 and SW480, While in Poorly Differentiated HCT116, The Protein shows Nuclear-Cytoplasmic Distribution Characteristics.

Article Snippet: A2BP1 Polyclonal antibody (Proteintech, USA, 22647-1-AP) was added dropwise and incubated at 37 °C for 1 h. The liquid was poured off, and the sections were rinsed with PBS three times.

Techniques: Immunofluorescence

Journal: Technology in Cancer Research & Treatment

Article Title: The Expression Characteristics of the RBFOX1 Gene in Colorectal Cancer

doi: 10.1177/15330338251333695

Figure Lengend Snippet: Immunohistochemical Analysis of RBFOX1 Expression Level in Normal and Cancerous Colorectal Tissues. A. Immunohistochemical Results from The Human Protein Atlas Database Show that the Protein is Mainly Distributed in the Glandular Cells of the Normal Intestine and shows Low Cytoplasmic Distribution Characteristics, But the Expression of this Protein is not Detected in Colorectal Cancer Tissues, Here are Two Representative Tissues. N1958 Representative an Immunohistochemical Results of RBFOX1 of 84 Years Old Female Patient in Normal Colon Tissue, 1958 is the Patient ID, N1857 Representative an Immunohistochemical Results of RBFOX1 of 47 Years Old Male Patient in Normal Colon Tissue, C4724 Representative an Immunohistochemical Results of RBFOX1 of 70 Years Old Female Patient in Adenocarcinoma Tissue, C4724 Representative an Immunohistochemical Results of RBFOX1 of 82 Years Old Female Patient in Colon Adenocarcinoma Tissue. ( https://www.proteinatlas.org/search/RBFOX1 ). B. Immunohistochemical Results of RBFOX1 in Two High-Throughput Pathological Tissue Chips (Consisting of 69 Colorectal Cancer Tissues). C. A Normal Brain Sample was Used as a Positive Control., On the Left is a 100x Microscopic Image of the Brain, And on the Right is a 200x Magnified Image of a Localized Area on the Left. D. Immunohistochemical Results of RBFOX1 in Intestinal Tissue from a Case of slow Transit Constipation Undergoing Total Colectomy. E. Immunohistochemical Results of RBFOX1 in Three Right-Sided Colorectal Cancers and Adjacent Tissues. N Means Normal, C Means Cancer.

Article Snippet: A2BP1 Polyclonal antibody (Proteintech, USA, 22647-1-AP) was added dropwise and incubated at 37 °C for 1 h. The liquid was poured off, and the sections were rinsed with PBS three times.

Techniques: Immunohistochemical staining, Expressing, High Throughput Screening Assay, Positive Control

Journal: Cell Death Discovery

Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke

doi: 10.1038/s41420-021-00773-w

Figure Lengend Snippet: A Representative microtubule-associated protein 2 (MAP2) immunostaining images of CRTC1 KO and WT neurons, before and after OGD. (Scale Bar, 100 μm). B LDH assay of CRTC1 KO and WT neuron up to 48 h after ischemia/reperfusion. Representative Western Blot images ( C ) and quantification ( D ) of RBFox-1 in CRTC1 KO and WT mice penumbra region. E Relative luciferase activity of CHO cell line co-transfected with RBFox-1 and miR-132 vehicle/mimic/antagomir. Representative Western Blot images ( F ) and quantification ( G ) of RBFox-1 in neurons transfected with miR-132 mimic/antagomir/vehicle, before and after OGD.

Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).

Techniques: Immunostaining, Lactate Dehydrogenase Assay, Western Blot, Luciferase, Activity Assay, Transfection

Journal: Cell Death Discovery

Article Title: MiRNA-132/212 regulates tight junction stabilization in blood–brain barrier after stroke

doi: 10.1038/s41420-021-00773-w

Figure Lengend Snippet: CRTC1-mediated miR-132/212 attenuates neuron damages via suppressing RBFox-1, and alleviates blood–brain barrier dysfunction by regulating TJAP-1 and Claudin-1 expression in ischemic stroke.

Article Snippet: The following antibodies were used: CRTC1 (1:1000; Cell Signaling Technology, Danvers, MA, USA); TJAP-1 (1:1000; Novus Biologicals); RBFox-1 (1:1000; Merck Millipore); E-Cadherin (1:1000; Cell Signaling Technology); Claudin-1 (1:1000; Invitrogen); cleaved Caspase-3 (1:1000; Cell Signaling Technology); β-actin (1:5000; Proteintech, Rosemont, IL, USA).

Techniques: Expressing